Effects Of Rats Exhaustive Swimming On Atp Synthesis Activity Of Kydney Mitochondria

Por: Bo Chang.

Athens 2004: Pre-olympic Congress

Send to Kindle


Introduction
In order to study the mechanisms of exercise-induced exhaustion and porteinuria, the experiment was designed to
investigate exhaustive exercise-induced oxidative stress on the functions of kidney mitochondria.

Methods
30 male Sprague-Dawley rats were randomly divided into four groups(control group, n=7; immediate postexercise
group, n=7; 1h postexercise group, n=8 and 24h postexercise group , n=8), and the changes were measured that the
content of MDA、ATP syntheses activity and total calcium in the kidney mitochondria in the different phases were
observed.

Results
The results are as follows: when compared with control group , the MDA content significantly rises immediately after
exercise(p<0.05)and it is the same to 1h and 24h postexercise group(p<0.01);The ATP synthesis activity of
kidney mitochondria of immediate postexercise, 1h postexercise group individually declines extremely as compared
with control group(p<0.01)while it does not recover to a normal value 24h after exercise; The mitochondria total
calcium of 1h and 24h groups is markedly higher than the control group (p<0.01; p<0.05).

Conclusions
The findings suggest that exhaustive swimming can lead to an increase of mitochondria lipid peroxidation , a decrease
of ATP synthesis activity of kidney mitochondria and irregularity of the steady state of the mitochondria calcium while
the mitochondria calcium accumulation probably results in the uncoupling of intramitochondria oxidative
phosphorylation and anormality of kidney functions. It might be one of the important reasons of exercise-induced
exhaustion and porteinuria.

References
[1].Bradford MM ,et al . A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the
principle of proteindye binding. Anal, Biochem. 1976;72:248-254
[2].Vaghy PL., et al Selective inhibition of Na+ induced Ca2+-release from heart mitochondria by diltiazem and
certain other antagonist drugs . J.Bio.Chem. 1982;257:6000-6002
[3].Krestzschmar M , et al Influence of aging, training and physical exercise on plasma glutathione and lipid peroxides
in man. Int. J. Sports Med. 1991;12:218-212
[4].Marx JI, Oxygen free radicals linked to many disease . Science . 1987;235(47):529
[5].Jenkins RR, Free radicals chemistry relationship to exercise . Sports Med. 1988;5:156
[6]. Lovlin R. Are indeces of free radical damage related to exercise intensity. Eur. J . Appl .Physiol , 1987;56:318
[7].Alessio HM. Lipid peroxidation and scavenger enzymes during exercise . J. Anim Sci, 1988;64:1333-1336
[8].Duan C , et al .Rat skeletal muscle mitochondrial Ca2+ and injury from downhill waking. J . Appl .Physiol .
1990;68:1241-1251
[9].Charles L. et al. The role of calcium in the pathogenesis of acute renal failure Renal Failure 1997;19(2 ):199-207
[10].Douglas R. et al . Mitochondrial calcium accumulation and respiration in ischemic acute renal failure in the rat
Kidney Int . 1984;25:519-526
[11]Cazzola R, Russo-Volpe S, Cervato G, Cestaro B. Biochemical assessments of oxidative stress, erythrocyte
membrane fluidity and antioxidant status in professional soccer players and sedentary controls. Eur J Clin Invest. 2003
Oct;33(10):924-30.
[12]Palazzetti S, Richard MJ, Favier A, Margaritis I. Overloaded training increases exercise-induced oxidative stress
and damage. Can J Appl Physiol. 2003 Aug;28(4):588-604.
[13]Wozniak A, Drewa G, Chesy G, Rakowski A, Rozwodowska M, Olszewska D. Effect of altitude training on the
peroxidation and antioxidant enzymes in sportsmen. Med Sci Sports Exerc. 2001 Jul;33(7):1109-13
[14]Nakano T, Wada Y, Matsumura S. Membrane lipid components associated with increased filterability of
erythrocytes from long-distance runners. Clin Hemorheol Microcirc. 2001;24(2):85-92.
[15]Tauler P, Gimeno I, Aguilo A, Guix MP, Pons A. Regulation of erythrocyte antioxidant enzyme activities in athletes
during competition and short-term recovery. Pflugers Arch. 1999 Nov;438(6):782-7.
[16]Kostka T, Drai J, Berthouze SE, Lacour JR, Bonnefoy M. Physical activity, fitness and integrated antioxidant
system in healthy active elderly women. Int J Sports Med. 1998 Oct;19(7):462-7.
[17]Kobe H, Nakai A, Koshino T, Araki T. Effect of regular maternal exercise on lipid peroxidation levels and
antioxidant enzymatic activities before and after delivery. J Nippon Med Sch. 2002 Dec;69(6):542-8.
[18]Vesovic D, Borjanovic S, Markovic S, Vidakovic A. Strenuous exercise and action of antioxidant enzymes. Med Lav.
2002 Nov-Dec;93(6):540-50.
[19]Tauler P, Gimeno I, Aguilo A, Guix MP, Pons A. Regulation of erythrocyte antioxidant enzyme activities in athletes
during competition and short-term recovery. Pflugers Arch. 1999 Nov;438(6):782-7.
[20]Avellini L, Chiaradia E, Gaiti A. Effect of exercise training, selenium and vitamin E on some free radical
scavengers in horses (Equus caballus). Comp Biochem Physiol B Biochem Mol Biol. 1999 Jun;123(2):147-54.
[21]Kaczmarski M, Wojcicki J, Samochowiec L, Dutkiewicz T, Sych Z. The influence of exogenous antioxidants and
physical exercise on some parameters associated with production and removal of free radicals. Pharmazie. 1999
Apr;54(4):303-6.
[22]Cazzola R, Russo-Volpe S, Cervato G, Cestaro B. Biochemical assessments of oxidative stress, erythrocyte
membrane fluidity and antioxidant status in professional soccer players and sedentary controls. Eur J Clin Invest. 2003
Oct;33(10):924-30.
[23]Palazzetti S, Richard MJ, Favier A, Margaritis I. Overloaded training increases exercise-induced oxidative stress
and damage. Can J Appl Physiol. 2003 Aug;28(4):588-604.
[24]Aguilo A, Tauler P, Pilar Guix M, Villa G, Cordova A, Tur JA, Pons A. Effect of exercise intensity and training on
antioxidants and cholesterol profile in cyclists. J Nutr Biochem. 2003 Jun;14(6):319-25.
[25]Di Meo S, Venditti P. Mitochondria in exercise-induced oxidative stress. Biol Signals Recept. 2001 Jan-Apr;10(1-
2):125-40.
[26]Lloyd PG, Yang HT, Terjung RL. Arteriogenesis and angiogenesis in rat ischemic hindlimb: role of nitric oxide. Am
J Physiol Heart Circ Physiol. 2001 Dec;281(6):H2528-38.
[27]Bolli R. Cardioprotective function of inducible nitric oxide synthase and role of nitric oxide in myocardial ischemia
and preconditioning: an overview of a decade of research. J Mol Cell Cardiol. 2001 Nov;33(11):1897-918.


NOTA: O texto com a iconografia está no anexo

Ver Arquivo (PDF)

Tags:

Comentários


:-)





© 1996-2022 Centro Esportivo Virtual - CEV.
O material veiculado neste site poderá ser livremente distribuído para fins não comerciais, segundo os termos da licença da Creative Commons.